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Work on what is now known as the Hydrophobic Subtraction
Model (HSM) was initiated by Dr. Lloyd Snyder and began
informally in the late 1990's. The extensive expertise in
and previous experience of Dr. Snyder and close
collaborators Dr. John Dolan, Dr. Uwe Neue, and Professors
Peter
Carr and Dan
Marchand with the subject of selectivity in HPLC
supported a vision for a broader understanding of
selectivity in Reversed-Phase HPLC (RPLC). This vision
subsequently turned into a long-term project that led to
the development of the HS model and characterization of
hundreds of RPLC columns. The early stages of this project
were supported financially by Phase I and II Small
Business Innovative Research (SBIR)
grants from the National Institutes of Health. The
project has been highly collaborative from its inception,
and has benefited immensely from involvement by innovators
in the pharmaceutical industry, many manufacturers of HPLC
columns, the Product Quality Research Institute (PQRI),
and the United States Pharmacopeia. A database containing
HS model parameters for hundreds of RPLC phases and an
associated tool for finding columns of similar or
different selectivity are also available at the USP
website.
In
late 2011, the work of characterizing of new stationary
phases transitioned from the BAS laboratory in Oregon to
the laboratory of Dr.
Dwight Stoll at Gustavus
College in Minnesota. Dr. Stoll currently maintains
the HS database and is pursuing further development of the
model with a number of collaborators. Column manufacturers
interested in having their stationary phases listed in the
HSM database should contact Dr. Stoll for more
information.
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