Welcome to hplccolumns.org - a free, online resource for selectivity data and tools for High Performance Liquid Chromatography.

A current database containing column parameters for over 700 reversed-phase HPLC columns characterized using the Hydrophobic Subtraction Model of reversed-phase selectivity can be found in the Column Selectivity Database tab.

In the How It Works tab, you will find background information about the fundamental basis of the Hydrophobic Subtraction Model.

See the About tab for information about the history of the Hydrophobic Subtraction Model and this website. We are very interested in feedback on both this site and the model, so please do not hesitate to contact us with comments or questions.

Today we have 7 new additions from several different vendors, bringing the database total to 751 columns.

Today we have 13 new additions from several different vendors, bringing the database total to 744 columns.

Today we have four new additions to the database - three from VDS Optilab of Germany, and one from YMC. This brings the total number of columns in the database to 737!

Today we have nine new additions to the database - three from GL Sciences of Japan, and six from Waters Corporation. This brings the total number of columns in the database to 714!

Three new additions each today from new contributing manufacturers Nanologica and Nanghavi Chromatography Solutions Ltd.

Three new additions from Phenomenex today bring the total number of columns to 698; we'll break 700 in 2017!

A lot has actually happened here since the last entry from 2014. With the addition of three new phases from Supelco into the database today, the total number columns stands at 695.

Today we've added an additional 40 columns to the database, bringing the current total to 589. As part of this update, we welcome new contributing manufacturers Orochem Technologies and Genius Technologies.

Today we've added an additional 14 new columns to the database, bringing the current total to 646. As part of this update, we welcome new contributing manufacturers Diamond Analytics and Nacalai Tesque.

Today we've added an additional 19 new columns to the database, bringing the current total to 665. As part of this update, we welcome new contributing manufacturers SIELC and ChromaNik.